Tuesday, January 21, 2014

In late 2010 a geological expedition to Antarctica drilled through the Ross Ice Shelf so they could send an ROV under it. What they found was unexpected: Sea anemones. In their thousands they were doing what no other species of sea anemone is known to do — they were living in the ice itself.

Discovered by the ANDRILL [Antarctic Drilling] project, the team was so unprepared for biological discoveries they did not have suitable preservatives and the only chemicals available obliterated the creature’s DNA. Nonetheless Marymegan Daly of Ohio State University confirmed the animals were a new species. Named Edwardsiella andrillae after the drilling project that found it, the anemone was finally described in a PLOS ONE paper last month.

ANDRILL lowered their cylindrical camera ROV down a freshly-bored 270m (890ft) hole, enabling it to reach seawater below the ice. The device was merely being tested ahead of its planned mission retrieving data on ocean currents and the sub-ice environment. Instead it found what ANDRILL director Frank Rack of the University of Nebraska–Lincoln, a co-author of the paper describing the find, called the “total serendipity” of “a whole new ecosystem that no one had ever seen before”.

The discovery raises many questions. Burrowing sea anemones worm their way into substrates or use their tentacles to dig, but it’s unclear how E. andrillae enters the hard ice. With only their tentacles protruding into the water from the underneath of the ice shelf questions also revolve around how the animals avoid freezing, how they reproduce, and how they cope with the continuously melting nature of their home. Their diet is also a mystery.

E. andrillae is an opaque white, with an inner ring of eight tentacles and twelve-to-sixteen tentacles in an outer ring. The ROV’s lights produced an orange glow from the creatures, although this may be produced by their food. It measures 16–20mm (0.6–0.8in) but when fully relaxed can extend to triple that.

Genetic analysis being impossible, Daly turned to dissection of the specimens but could find nothing out of the ordinary. Scientists hope to send a biological mission to explore the area under the massive ice sheet, which is in excess of 600 miles (970km) wide. The cameras also observed worms, fish that swim inverted as if the icy roof was the sea floor, crustaceans and a cylindrical creature that used appendages on its ends to move and to grab hold of the anemones.

NASA is providing funding to aid further research, owing to possible similarities between this icy realm and Europa, a moon of Jupiter. Biological research is planned for 2015. An application for funding to the U.S. National Science Foundation, which funds ANDRILL, is also pending.

The ANDRILL team almost failed to get any samples at all. Designed to examine the seafloor, the ROV had to be inverted to examine the roof of ice. Weather conditions prevented biological sampling equipment being delivered from McMurdo Station, but the scientists retrieved 20–30 anemones by using hot water to stun them before sucking them from their burrows with an improvised device fashioned from a coffee filter and a spare ROV thruster. Preserved on-site in ethanol, they were taken to McMurdo station where some were further preserved with formaldehyde.

((Wikinews)) How did you come to be involved with this discovery?

Marymegan Daly: Frank Rack got in touch after they returned from Antarctica in hopes that I could help with an identification on the anemone.

((Wikinews)) What was your first reaction upon learning there was an undiscovered ecosystem under the ice in the Ross Sea?

MD I was amazed and really excited. I think to say it was unexpected is inaccurate, because it implies that there was a well-founded expectation of something. The technology that Frank and his colleagues are using to explore the ice is so important because, given our lack of data, we have no reasonable expectation of what it should be like, or what it shouldn’t be like.

((Wikinews)) There’s a return trip planned hopefully for 2015, with both biologists and ANDRILL geologists. Are you intending to go there yourself?

MD I would love to. But I am also happy to not go, as long as someone collects more animals on my behalf! What I want to do with the animals requires new material preserved in diverse ways, but it doesn’t require me to be there. Although I am sure that being there would enhance my understanding of the animals and the system in which they live, and would help me formulate more and better questions about the anemones, ship time is expensive, especially in Antarctica, and if there are biologists whose contribution is predicated on being there, they should have priority to be there.

((Wikinews)) These animals are shrouded in mystery. Some of the most intriguing questions are chemical; do they produce some kind of antifreeze, and is that orange glow in the ROV lights their own? Talk us through the difficulties encountered when trying to find answers with the specimens on hand.

MD The samples we have are small in terms of numbers and they are all preserved in formalin (a kind of formaldehyde solution). The formalin is great for preserving structures, but for anemones, it prevents study of DNA or of the chemistry of the body. This means we can’t look at the issue you raise with these animals. What we could do, however, was to study anatomy and figure out what it is, so that when we have samples preserved for studying e.g., the genome, transcriptome, or metabolome, or conduct tests of the fluid in the burrows or in the animals themselves, we can make precise comparisons, and figure out what these animals have or do (metabolically or chemically) that lets them live where they live.

Just knowing a whole lot about a single species isn’t very useful, even if that animal is as special as these clearly are — we need to know what about them is different and thus related to living in this strange way. The only way to get at what’s different is to make comparisons with close relatives. We can start that side of the work now, anticipating having more beasts in the future.

In terms of their glow, I suspect that it’s not theirs — although luminescence is common in anemone relatives, they don’t usually make light themselves. They do make a host of florescent proteins, and these may interact with the light of the ROV to give that gorgeous glow.

((Wikinews)) What analysis did you perform on the specimens and what equipment was used?

MD I used a dissecting scope to look at the animal’s external anatomy and overall body organization (magnification of 60X). I embedded a few of the animals in wax and then cut them into very thin slices using a microtome, mounted the slices on microscope slides, stained the slices to enhance contrast, and then looked at those slides under a compound microscope (that’s how I got the pictures of the muscles etc in the paper). I used that same compound scope to look at squashed bits of tissue to see the stinging capsules (=nematocysts).

I compared the things I saw under the ‘scopes to what had been published on other species in this group. This step seems trivial, but it is really the most important part! By comparing my observations to what my colleagues and predecessors had found, I figured out what group it belongs to, and was able to determine that within that group, it was a new species.

((Wikinews)) It was three years between recovery of specimens and final publication, why did it take so long?

MD You mean, how did we manage to make it all happen so quickly, right? 🙂 It was about two years from when Frank sent me specimens to when we got the paper out. Some of that time was just lost time — I had other projects in the queue that I needed to finish. Once we figured out what it was, we played a lot of manuscript email tag, which can be challenging and time consuming given the differing schedules that folks keep in terms of travel, field work, etc. Manuscript review and processing took about four months.

((Wikinews)) What sort of difficulties were posed by the unorthodox preservatives used, and what additional work might be possible on a specimen with intact DNA?

MD The preservation was not unorthodox — they followed best practices for anatomical preservation. Having DNA-suitable material will let us see whether there are new genes, or genes turned on in different ways and at different times that help explain how these animals burrow into hard ice and then survive in the cold. I am curious about the population structure of the “fields” of anemones — the group to which Edwardsiella andrillae belongs includes many species that reproduce asexually, and it’s possible that the fields are “clones” produced asexually rather than the result of sexual reproduction. DNA is the only way to test this.

((Wikinews)) Do you have any theories about the strategies employed to cope with the harsh environment of burrowing inside an ice shelf?

MD I think there must be some kind of antifreeze produced — the cells in contact with ice would otherwise freeze.

((Wikinews)) How has such an apparently large population of clearly unusual sea anemones, not to mention the other creatures caught on camera, gone undetected for so long?

MD I think this reflects how difficult it is to get under the ice and to collect specimens. That being said, since the paper came out, I have been pointed towards two other reports that are probably records of these species: one from Japanese scientists who looked at footage from cameras attached to seals and one from Americans who dove under ice. In both of these cases, the anemone (if that’s what they saw) was seen at a distance, and no specimens were collected. Without the animals in hand, or the capability of a ROV to get close up for pictures, it is hard to know what has been seen, and lacking a definitive ID, hard to have the finding appropriately indexed or contextualized.

((Wikinews)) Would it be fair to say this suggests there may be other undiscovered species of sea anemone that burrow into hard substrates such as ice?

MD I hope so! What fascinates me about sea anemones is that they’re able to do things that seem impossible given their seemingly limited toolkit. This finding certainly expands the realm of possible.

Wednesday, May 16, 2007

New York City Mayor Michael Bloomberg is prepared to spend one billion dollars of his own money for a 2008 White House bid, the Washington Times reports, citing his friends and “close associates” .

However, Mayor Bloomberg yesterday made light of Senator Chuck Hagel‘s suggestion that Bloomberg could run as an independent with Hagel as running mate. Bloomberg has so far denied that he is running but has not ruled out the possibility either.

Media reports speculate that Bloomberg is considering a run if the Democratic and Republican nominees cater more to the parties’ bases, leaving room for an independent candidate who can attract moderate voters for both camps.

TIME magazine reports that Bloomberg was at first ready to consider spending US$500 million for a presidential run.

Michael Bloomberg could have more money than either party candidates, former FEC chairman Michael E. Toner told the Times. Those funds would come in handy to mobilize the thousands of petitions needed to qualify for a run in some states.

The Washington Times also has reports of a Bloomberg adviser saying some people involved with John McCain‘s Presidential Campaign are prepared to leave and join Bloomberg’s campaign if he does indeed run.

Bloomberg’s advisers have also said that they have been studying and learning from Ross Perot‘s Presidential campaign in 1992. They have called and talked with Perot’s former advisers and campaign workers to learn from their mistakes and experience.

Bloomberg made his fortune with his company Bloomberg L.P., a financial news and data company. Forbes magazine ranked him 44th on a list of wealthiest Americans and 142nd on a list of billionaires worldwide. His net worth is estimated at US$5.5 billion.

Friday, March 18, 2011

A power line to the Fukushima I Nuclear Power Plant in Japan was completed Thursday, which will allow cooling systems at one of the facility’s reactors.

The new line, roughly one kilometer (0.6 miles) in length, connects the power grid to the power plant’s second reactor. It was completed at about 1730 local time (0830 UTC) on Thursday, and will be energized once spraying of seawater over reactor three is complete.

According to a Tokyo Electric Power Company spokesperson, once the power line is energized “we will be able to activate various electric pumps and pour water into reactors and pools for spent nuclear fuel,” thus cooling temperatures within the reactor.

The International Atomic Energy Agency has said that the stricken power plant is currently stable, though there is still the chance of matters growing worse. An official at the agency said that “it’s reasonably stable at the moment compared to yesterday.”

Saturday, September 18, 2010

Chile is celebrating its Bicentennial, with several events that have been organized by the government for almost a decade. It commemorates two hundred years since the First Government Junta of 1810 was formed, starting the Independence process, that ended in 1818 after Bernardo O’Higgins proclaimed it.

The Bicentennial takes place on a holiday from September 17th until 21st. Sebastián Piñera inaugurated the official fondas (places where typical food and drinks of Chile are sold; similar to a tavern) earlier on Friday. Piñera also danced a “pie” of Cueca, Chile’s national dance, with Government Spokeswoman Ena Von Baer.

More than 60 thousand people gathered on Plaza de la Ciudadanía (Citizen’s Square) in Santiago to celebrate the Bicentennial. There was a projection of historical images that also contained a message from the trapped miners in Copiapó. A giant flag of Chile (18 meters of height, 27 of width; weighing 200 kilograms) was raised on the square on Friday morning.

Celebrations of the Bicentennial in Pichilemu started earlier this month. On September 2, two thousand people lined up in a formation to create the message “Viva Chile Bicentenario Cardenal Caro” on Pichilemu beach “Las Terrazas”. The message was used to create a postal stamp to be released worldwide. The event was promoted by the Government of Cardenal Caro Province.

Private schools in the city, such as Colegio Preciosa Sangre, prepared events specially for their students. On Thursday, “Fonda Don Vicente Nario” was opened on Preciosa Sangre. Several games were performed there on that morning, including “el emboque”, “ponerle la cola al burro” (to put the tail to the donkey), and others.

Another event on Preciosa Sangre took place on Thursday night, when students recreated scenes of the History of Chile, including: a tertulia featuring Manuel Montt (starred by Luis Rojas); a chingana (a popular tavern); and selected colonial professions, such as the “motero” (person who sold motemei and chestnuts).

The official fonda of Pichilemu, La Bombonera, was inaugurated on Thursday night by Mayor Roberto Córdova, who danced cueca with people who attended the event. According to Córdova, at least 30,000 people have arrived at Pichilemu as of Friday, and it is estimated that another 30,000 will arrive during the next three days.

A great event took place on Pichilemu beach on Friday afternoon. Chilean typical games highlighted the event. People danced reggaeton, Américo’s cumbias and cuecas, while others were swimming. The National Shoe Fair (Feria Nacional del Calzado) was established on Agustín Ross Hotel on Thursday, and will stay in the town until September 23rd. Alicia Grez, who works on a kiosk in the Pichileminian Craft Fair located in front of One Discotheque, said that “sales have been excellent,” and that “[they] won’t miss the possibility to experience such an event like this.”

Friday, May 6, 2005

On Thursday the 24th of March, 2005, Anna Ayala, a woman from Las Vegas, claimed to have found a human finger in her bowl of chili at a Wendy’s restaurant located at 1405 Monterey Highway, just south of downtown San Jose, California, owned by Fresno-based Jern Management. The finger, which probably belonged to a woman as it had a long and manicured fingernail, did not belong to any of the restaurant employees. The food supplies were seized by officials to be traced back to its manufacturers, while the restaurant was permitted to open again later with chili prepared from fresh ingredients.

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Wikinews reporter David Vasquez drove his car up to the drive-thru menu and found that chili was still on the menu, at a price of US$1.19 for a small serving. He also witnessed workers unloading supplies from a semi-trailer truck in the restaurant’s parking lot, and carting them into the back door of the establishment.

Initially, county health officials said Ayala was fine and the finger had been cooked, which would have killed any bacteria in the finger. However, on March 27, officials admitted they were not so sure anymore. Tests were done on the finger to determine this. Dr. Martin Fenstersheib, Santa Clara County’s health officer, said that even if the finger was still raw when Ayala bit into it, the risk was low that she would have become infected with anything. However, he advised that Ayala should undergo a series of precautionary follow-up tests.

Sales at Wendy’s went down because of the incident. Wendy’s International, Inc. (WEN) closed at US$39.43 on Thursday the 24th, and as the stock exchange was closed for the Good Friday holiday, traders did not weigh in the stock until the next Monday.

By Tuesday the 5th of April, officials had still not succeeded in tracking down the owner of the finger. The fingerprint on the detached digit has been run through an FBI database as well as the local criminal database in Santa Clara County, but no matches were found. According to Rich Reneau, who was leading the investigation at the time, the fingerprint was marginal, and the likelihood of finding a match was slim.

Wendy’s stock did not go down significantly and was trading at US$39.37 that morning.

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The next day, on Wednesday the 6th, Las Vegas police searched the home of Anna Ayala. About a dozen officers conducted the search at Ayala’s home at Maryland Parkway and Serene Street at about 4 p.m. local time (23:00 UTC), according to witnesses at the scene. Ayala and other residents were handcuffed and brought out of the house. Ayala said that her teenage daughter, Genesis Reyes, had torn shoulder ligaments as a result of the search. The Las Vegas Review-Journal ran a photo of Reyes wearing a sling in their Friday edition. In San Jose, police spokeswoman Gina Tepoorten confirmed to reporters that investigators had served the warrant in cooperation with Las Vegas police on Wednesday, but she refused to reveal specific details about the warrant. By that time, Wendy’s was offering a US$50,000 reward for information leading to the source of the finger.

Research by the Associated Press uncovered Ayala’s history of lawsuits. Ayala successfully won her suit for medical expenses against the national El Pollo Loco chicken-chain, a previous employer, after her daughter Genesis contracted salmonella poisoning, allegedly from eating at the restaurant. However, Ayala lost another suit against General Motors in 2000 claiming that a wheel fell off her car. She also started a sexual harassment suit against her former boss in 1998. A total of 13 lawsuits in California and Nevada had been filed. Ayala replied the focus should be on Wendy’s, and not her record of lawsuits. Nick Muyo, a spokesman for the San Jose Police department, said not to expect new information in the case for at least a week.

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On Wednesday the 13th there was a potential new lead in the investigation. A spotted leopard had torn off part of a finger from an owner of exotic animals, Sandy Allman, in Pahrump, Nevada. The portion of Allman’s torn off finger was approximately the same size – 1 1/2-inches long. Pahrump is approximately 45 miles away from Las Vegas. Carol Asvestas, who owns an exotic animal sanctuary, told the San Jose Mercury News she witnessed the leopard tear off the finger. She reported the incident to a hotline run by Wendy’s offering the US$50,000 reward. Cindy Carroccio told the San Jose Mercury News that the finger was not reattached, and that the clinic “gave it back to her (Allman) in a little bag of ice.” On the same day the lead was announced, Ayala decided to drop her lawsuit against Wendy’s, due to emotional stress.

However, when Allman’s prints were sent to San Jose police, they didn’t match. Two days later, on Friday the 15th, Wendy’s doubled the reward to US$100,000. The company revealed that employees had passed polygraph tests. Wendy’s continues to claim that there is no evidence that the finger ever entered their supply chain, pointing to a lack of any accidents among the workers at their suppliers. Wendy’s tip line had received reports from across the United States, from “folks who either have lost a finger, or know somebody who lost a finger,” San Jose police Sgt. Nick Muyo told the Associated Press.

[edit]

On Thursday the 21st of April, Anna Ayala was arrested at or near her home in Las Vegas on Thursday evening, in connection with the case, shortly after Wendy’s finished its own internal investigation. According to court documents, she has been charged with one count of attempted grand larceny related to the chili case, and one count of grand larceny in an unrelated real estate deal, and is being held without bail in Clark County, Nevada, pending extradition. A press conference by the San Jose Police and Wendy’s was held on Friday, April 22, at 13:00 PDT. The charge related to the case states the finger could not have been prepared at Wendy’s, where the chili is heated to 170 degrees for 3 hours. There is also an inconsistency in Ayala’s account of finding the finger and claiming it caused her to vomit compared with police saying there was no vomit at the scene. The incident has caused Wendy’s 2.5 million dollars worth of damages, which Ayala could be criminally responsible for. Until recently, the San Jose police had not accused Ayala of planting the finger herself.

The unrelated charge stems from an incident, also in San Jose, when Ayala allegedly received an $11,000 down payment on a mobile home she did not own.

Ayala was incarcerated at the Clark County Detention Center, awaiting a fugitive review hearing on Tuesday, April 26, 2005, at 7:30 a.m. local time. She was processed and given inmate ID 01964047. Her case number was 05F07229X. Ayala waived extradition at the hearing, and her attorney said they were ready to come to San Jose to defend against the charges.

[edit]

On Friday, May 6, 2005, Ayala was transported to San Jose, California. Ayala was booked into the main Santa Clara County jail, and is awaiting arraignment. Ayala will likely be arraigned on Monday or Tuesday at the Santa Clara County Superior Court, according to Santa Clara County Deputy District Attorney David Boyd.

On September 9, 2005, Ayala pleaded guilty to conspiring to file a false claim and attempted grand theft, and was scheduled to be sentenced on November 2, 2005. She faced up to ten years in prison, and her husband faced up to 13 years behind bars.

[edit]

Until the middle of May, the owner of the finger still had not been found.

But on May 13, 2005, police announced that they had identified the finger tip as belonging to an associate of Ayala’s husband [1]. The associate had lost his finger tip in an industrial accident at an asphalt company[2] in December, 2004. Police had received the information from an undisclosed caller to Wendy’s hot-line.

Photos related to this incident:

• The Wendy’s location where the finger was discovered, shortly after closing time March 22

• Chili was still on the menu as the restaurant closed for the evening on March 22

• Workers unload fresh supplies after closing time on March 22

• The Wendy’s location on March 27

• Ayala residence in Las Vegas, Nevada, shot on April 22, 2005 by Wikinews contributor David Vasquez

• Note on Ayala residence door, photographed on April 22, 2005 by David Vasquez

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